KMID : 0903519900330040354
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology 1990 Volume.33 No. 4 p.354 ~ p.355
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Microbial degradation of th persistent pollutnat TCAB
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Lee, Jae Koo
Kyung, Kee Sung/Oh, Kyeong Seok/lhm, Yang Bin/Cho, Yong Gyun/Kim, Nam
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Abstract
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In order to investigate the possibility of the microbial degradation of the persistent pollutant TCAB(3, 3¢¥, 4, 4¢¥-tetrachloroazobenzene) in our environment, four strains of microorganisms were isolated from industrial wastes by the enrichment technique. They were identified as Achromobacter group VD, Pseudomonas testosteroni/alcaligenes, Maraxella spp., and Alcaligenes faecalis, respectively. These microorganisms utilized TCAB as a sole carbon source in the MM©ü salt medium. When ^(14)C-TCAB was added to the medium as a sole carbon source and incubated, some radioactive metabolites were detected by autoradiography.
No ^(14)CO©ü was evolved from ^(14)C-TCAB which was added as a sole carbon source to an organic matter-free soil inoculated by one of the isolates(Achromobacter group VD), wetted with the MM©ü medium, and incubated at 30¡É.
One of the metabolites in pure culture of these microorganisms was tentatively identified as a compound of m/z 250 by means of GC/MS.
The possible pathways for its formation are thought to include dechlorination from TCAB structure, hydroxylation, ortho fission of the two benzene rings, and reduction of the resulting carboxyl group.
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